pCMV6-AC-IRES-GFP
PrecisionShuttle™ mammalian expression vector with a TurboGFP marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags.
Sequence Author: OriGene
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Sticky ends from different AlwNI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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