pCMV-DDK-C

Mammalian vector for CMV promoter-driven expression of an ORF with a C-terminal FLAG® tag.

Sequence Author: transOMIC

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
EcoRI (832) SalI (839) AccI (840) BglII (847) AvaI - BmeT110I - BsoBI - PaeR7I - XhoI (853) Acc65I (858) KpnI (862) FLAG EagI - NotI (895) AanI (1019) M13 rev pCMV-DDK-C 3766 bp
EcoRI  (832)
1 site
G A A T T C C T T A A G
SalI  (839)
1 site
G T C G A C C A G C T G
AccI  (840)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BglII  (847)
1 site
A G A T C T T C T A G A
AvaI  (853)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (853)
1 site
C Y C G R G G R G C Y C
BsoBI  (853)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (853)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (853)
1 site
C T C G A G G A G C T C
Acc65I  (858)
1 site
G G T A C C C C A T G G
KpnI  (862)
1 site
G G T A C C C C A T G G
EagI  (895)
1 site
C G G C C G G C C G G C
NotI  (895)
1 site
G C G G C C G C C G C C G G C G
AanI  (1019)
1 site
T T A T A A A A T A T T
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2311 .. 3102  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3103 .. 3171  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2311 .. 3171  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1552 .. 2140  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
1552 .. 2140  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
905 .. 1039  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
905 .. 1039  =  135 bp
SV40 polyadenylation signal
AmpR promoter
3172 .. 3276  =  105 bp
AmpR promoter
3172 .. 3276  =  105 bp
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor sites
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor sites
MCS
832 .. 863  =  32 bp
multiple cloning site
MCS
832 .. 863  =  32 bp
multiple cloning site
FLAG
867 .. 890  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
867 .. 890  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
M13 rev
1150 .. 1166  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
1150 .. 1166  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  2441 .. 2707  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3557 .. 36  =  246 bp
ORF:  81 amino acids  =  9.5 kDa
ORF:  784 .. 1056  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  2311 .. 3171  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3517 .. 113  =  363 bp
ORF:  120 amino acids  =  13.4 kDa
Click here to try SnapGene

Download pCMV-DDK-C.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.