pFN22A HaloTag CMVd1

Flexi® vector with an ampicillin resistance marker, for mammalian expression from a slightly compromised CMV promoter of a protein with an N-terminal HaloTag®.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
BsaBI * (4182) MluI (4064) BspEI (3851) PciI (3087) AgeI (3019) BstBI (2992) AhdI (2917) AseI (2742) FspI (2694) XmnI (2317) SspI (2112) BglII (4183) HindIII (127) PflMI (232) AleI (290) BstEII (308) BseRI (310) PflFI - Tth111I (440) BmgBI (442) PasI (472) StuI * (641) BclI * (672) BspDI * - ClaI * (678) PshAI (737) SgrAI (824) BbsI (1023) BssHII (1049) PaeR7I - XhoI (1067) AsiSI - PvuI - SgfI (1125) BtgI - NcoI (1128) DraI - PmeI (1492) Acc65I (1509) KpnI (1513) XbaI (1524) PstI - SbfI (1540) BfuAI - BspMI (1543) BlpI (1605) NotI (1635) AanI - PsiI (1758) HpaI (1778) MfeI (1787) SnaBI (1979) pFN22A HaloTag® CMVd1 4188 bp
BsaBI  (4182)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MluI  (4064)
1 site
A C G C G T T G C G C A
BspEI  (3851)
1 site
T C C G G A A G G C C T
PciI  (3087)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (3019)
1 site
A C C G G T T G G C C A
BstBI  (2992)
1 site
T T C G A A A A G C T T
AhdI  (2917)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AseI  (2742)
1 site
A T T A A T T A A T T A
FspI  (2694)
1 site
T G C G C A A C G C G T
XmnI  (2317)
1 site
G A A N N N N T T C C T T N N N N A A G
SspI  (2112)
1 site
A A T A T T T T A T A A
BglII  (4183)
1 site
A G A T C T T C T A G A
HindIII  (127)
1 site
A A G C T T T T C G A A
PflMI  (232)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (290)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (308)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BseRI  (310)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflFI  (440)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (440)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmgBI  (442)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PasI  (472)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
StuI  (641)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BclI  (672)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspDI  (678)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (678)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PshAI  (737)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SgrAI  (824)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BbsI  (1023)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BssHII  (1049)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PaeR7I  (1067)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1067)
1 site
C T C G A G G A G C T C
AsiSI  (1125)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1125)
1 site
C G A T C G G C T A G C
SgfI  (1125)
1 site
G C G A T C G C C G C T A G C G
BtgI  (1128)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1128)
1 site
C C A T G G G G T A C C
DraI  (1492)
1 site
T T T A A A A A A T T T
PmeI  (1492)
1 site
G T T T A A A C C A A A T T T G
Acc65I  (1509)
1 site
G G T A C C C C A T G G
KpnI  (1513)
1 site
G G T A C C C C A T G G
XbaI  (1524)
1 site
T C T A G A A G A T C T
PstI  (1540)
1 site
C T G C A G G A C G T C
SbfI  (1540)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (1543)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1543)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (1605)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (1635)
1 site
G C G G C C G C C G C C G G C G
AanI  (1758)
1 site
T T A T A A A A T A T T
PsiI  (1758)
1 site
T T A T A A A A T A T T
HpaI  (1778)
1 site
G T T A A C C A A T T G
MfeI  (1787)
1 site
C A A T T G G T T A A C
SnaBI  (1979)
1 site
T A C G T A A T G C A T
HaloTag®
191 .. 1081  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
HaloTag®
191 .. 1081  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
TEV site
1094 .. 1114  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
TEV site
1094 .. 1114  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
AmpR
2130 .. 2990  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2130 .. 2198  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2130 .. 2990  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2199 .. 2990  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2130 .. 2990  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
3148 .. 3736  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3148 .. 3736  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
1152 .. 1487  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
1152 .. 1487  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
3852 .. 4135  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
3852 .. 4135  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
SV40 poly(A) signal
1657 .. 1778  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1657 .. 1778  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2025 .. 2129  =  105 bp
AmpR promoter
2025 .. 2129  =  105 bp
CMVd1 promoter
1 .. 100  =  100 bp
truncated human cytomegalovirus (CMV) immediate early promoter that yields slightly reduced expression relative to the full-length promoter
CMVd1 promoter
1 .. 100  =  100 bp
truncated human cytomegalovirus (CMV) immediate early promoter that yields slightly reduced expression relative to the full-length promoter
T7 promoter
132 .. 150  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
132 .. 150  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
156 .. 174  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
156 .. 174  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  191 .. 1138  =  948 bp
ORF:  315 amino acids  =  35.7 kDa
ORF:  1883 .. 2233  =  351 bp
ORF:  116 amino acids  =  13.1 kDa
ORF:  4097 .. 178  =  270 bp
ORF:  89 amino acids  =  10.2 kDa
ORF:  1152 .. 1487  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  2130 .. 2990  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  754 .. 990  =  237 bp
ORF:  78 amino acids  =  8.0 kDa
ORF:  969 .. 1208  =  240 bp
ORF:  79 amino acids  =  8.9 kDa
ORF:  158 .. 1153  =  996 bp
ORF:  331 amino acids  =  37.5 kDa
ORF:  2594 .. 2860  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pFN22A HaloTag CMVd1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.