pFN24A HaloTag CMVd3

Flexi® vector with an ampicillin resistance marker, for mammalian expression from a strongly compromised CMV promoter of a protein with an N-terminal HaloTag®.

Sequence Author: Promega

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BsaBI * (4127) MluI (4009) BspEI (3796) PciI (3032) AgeI (2964) BstBI (2937) AhdI (2862) AseI (2687) FspI (2639) XmnI (2262) SspI (2057) BglII (4128) HindIII (72) PflMI (177) AleI (235) BstEII (253) BseRI (255) PflFI - Tth111I (385) BmgBI (387) PasI (417) StuI * (586) BclI * (617) BspDI * - ClaI * (623) PshAI (682) SgrAI (769) BbsI (968) BssHII (994) PaeR7I - XhoI (1012) AsiSI - PvuI - SgfI (1070) BtgI - NcoI (1073) DraI - PmeI (1437) Acc65I (1454) KpnI (1458) XbaI (1469) PstI - SbfI (1485) BfuAI - BspMI (1488) BlpI (1550) NotI (1580) AanI - PsiI (1703) HpaI (1723) MfeI (1732) SnaBI (1924) pFN24A HaloTag® CMVd3 4133 bp
BsaBI  (4127)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MluI  (4009)
1 site
A C G C G T T G C G C A
BspEI  (3796)
1 site
T C C G G A A G G C C T
PciI  (3032)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (2964)
1 site
A C C G G T T G G C C A
BstBI  (2937)
1 site
T T C G A A A A G C T T
AhdI  (2862)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AseI  (2687)
1 site
A T T A A T T A A T T A
FspI  (2639)
1 site
T G C G C A A C G C G T
XmnI  (2262)
1 site
G A A N N N N T T C C T T N N N N A A G
SspI  (2057)
1 site
A A T A T T T T A T A A
BglII  (4128)
1 site
A G A T C T T C T A G A
HindIII  (72)
1 site
A A G C T T T T C G A A
PflMI  (177)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (235)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (253)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BseRI  (255)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflFI  (385)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (385)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmgBI  (387)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PasI  (417)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
StuI  (586)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BclI  (617)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspDI  (623)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (623)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PshAI  (682)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SgrAI  (769)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BbsI  (968)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BssHII  (994)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PaeR7I  (1012)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1012)
1 site
C T C G A G G A G C T C
AsiSI  (1070)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1070)
1 site
C G A T C G G C T A G C
SgfI  (1070)
1 site
G C G A T C G C C G C T A G C G
BtgI  (1073)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1073)
1 site
C C A T G G G G T A C C
DraI  (1437)
1 site
T T T A A A A A A T T T
PmeI  (1437)
1 site
G T T T A A A C C A A A T T T G
Acc65I  (1454)
1 site
G G T A C C C C A T G G
KpnI  (1458)
1 site
G G T A C C C C A T G G
XbaI  (1469)
1 site
T C T A G A A G A T C T
PstI  (1485)
1 site
C T G C A G G A C G T C
SbfI  (1485)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (1488)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1488)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (1550)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (1580)
1 site
G C G G C C G C C G C C G G C G
AanI  (1703)
1 site
T T A T A A A A T A T T
PsiI  (1703)
1 site
T T A T A A A A T A T T
HpaI  (1723)
1 site
G T T A A C C A A T T G
MfeI  (1732)
1 site
C A A T T G G T T A A C
SnaBI  (1924)
1 site
T A C G T A A T G C A T
HaloTag®
136 .. 1026  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
HaloTag®
136 .. 1026  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
TEV site
1039 .. 1059  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
TEV site
1039 .. 1059  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
AmpR
2075 .. 2935  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2075 .. 2143  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2075 .. 2935  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2144 .. 2935  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2075 .. 2935  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
3093 .. 3681  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3093 .. 3681  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
1097 .. 1432  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
1097 .. 1432  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
3797 .. 4080  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
3797 .. 4080  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
SV40 poly(A) signal
1602 .. 1723  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1602 .. 1723  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1970 .. 2074  =  105 bp
AmpR promoter
1970 .. 2074  =  105 bp
CMVd3 promoter
1 .. 45  =  45 bp
truncated human cytomegalovirus (CMV) immediate early promoter that yields strongly reduced expression relative to the full-length promoter
CMVd3 promoter
1 .. 45  =  45 bp
truncated human cytomegalovirus (CMV) immediate early promoter that yields strongly reduced expression relative to the full-length promoter
T7 promoter
77 .. 95  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
77 .. 95  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
101 .. 119  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
101 .. 119  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  136 .. 1083  =  948 bp
ORF:  315 amino acids  =  35.7 kDa
ORF:  1828 .. 2178  =  351 bp
ORF:  116 amino acids  =  13.1 kDa
ORF:  1097 .. 1432  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  2075 .. 2935  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  699 .. 935  =  237 bp
ORF:  78 amino acids  =  8.0 kDa
ORF:  914 .. 1153  =  240 bp
ORF:  79 amino acids  =  8.9 kDa
ORF:  103 .. 1098  =  996 bp
ORF:  331 amino acids  =  37.5 kDa
ORF:  2539 .. 2805  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pFN24A HaloTag CMVd3.dna file

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