pTracer-CMV2

Mammalian vector with a CMV promoter, for co-expression with GFP fused to a Zeocin™ resistance marker. See also pTracer™-CMV.

Sequence Author: Thermo Fisher (Invitrogen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
PmeI (904) Acc65I (917) KpnI (921) BstXI (948) EcoRI (952) EcoRV (964) BstXI (974) NotI (979) XbaI (991) PmeI (1006) pTracer™-CMV2 6210 bp
PmeI  (904)
2 sites
G T T T A A A C C A A A T T T G
Acc65I  (917)
1 site
G G T A C C C C A T G G
KpnI  (921)
1 site
G G T A C C C C A T G G
BstXI  (948)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (952)
1 site
G A A T T C C T T A A G
EcoRV  (964)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (974)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (979)
1 site
G C G G C C G C C G C C G G C G
XbaI  (991)
1 site
T C T A G A A G A T C T
PmeI  (1006)
2 sites
G T T T A A A C C A A A T T T G
EF-1α promoter
1309 .. 2487  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
1309 .. 2487  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
Cycle 3 GFP
2677 .. 3375  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2677 .. 3375  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
BleoR
3376 .. 3753  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
3376 .. 3753  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
AmpR
5214 .. 6074  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5214 .. 6005  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5214 .. 6074  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6006 .. 6074  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5214 .. 6074  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4455 .. 5043  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4455 .. 5043  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3883 .. 4004  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3883 .. 4004  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
1028 .. 1139  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1028 .. 1139  =  112 bp
bovine growth hormone polyadenylation signal
MCS
901 .. 1010  =  110 bp
multiple cloning site
MCS
901 .. 1010  =  110 bp
multiple cloning site
AmpR promoter
6075 .. 6179  =  105 bp
AmpR promoter
6075 .. 6179  =  105 bp
EM7 promoter
2611 .. 2658  =  48 bp
synthetic bacterial promoter
EM7 promoter
2611 .. 2658  =  48 bp
synthetic bacterial promoter
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2504 .. 2522  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2504 .. 2522  =  19 bp
promoter for bacteriophage T7 RNA polymerase
EF-1α intron A
1540 .. 2478  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
1540 .. 2478  =  939 bp
intron upstream of the start codon of human EF-1α
ORF:  2677 .. 3753  =  1077 bp
ORF:  358 amino acids  =  40.0 kDa
ORF:  5344 .. 5610  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1865 .. 2302  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  1944 .. 2177  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  3276 .. 3659  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
ORF:  5214 .. 6074  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1607 .. 2227  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
Click here to try SnapGene

Download pTracer-CMV2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.