pTracer-EF V5-His A

Mammalian vector with an EF-1α promoter, for co-expression with GFP fused to a Zeocin™ resistance marker. See also pTracer™-EF/V5-His B and pTracer™-EF/V5-His C.

Sequence Author: Thermo Fisher (Invitrogen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
Acc65I (1715) KpnI (1719) SpeI (1733) BstXI (1746) EcoRI (1750) EcoRV (1762) BstXI (1772) NotI (1777) XbaI (1789) 6xHis PmeI (1882) pTracer™-EF/V5-His A 5948 bp
Acc65I  (1715)
1 site
G G T A C C C C A T G G
KpnI  (1719)
1 site
G G T A C C C C A T G G
SpeI  (1733)
1 site
A C T A G T T G A T C A
BstXI  (1746)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (1750)
1 site
G A A T T C C T T A A G
EcoRV  (1762)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstXI  (1772)
2 sites
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (1777)
1 site
G C G G C C G C C G C C G G C G
XbaI  (1789)
1 site
T C T A G A A G A T C T
PmeI  (1882)
1 site
G T T T A A A C C A A A T T T G
V5 tag
1807 .. 1848  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
1807 .. 1848  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
1858 .. 1875  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
1858 .. 1875  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
EF-1α promoter
475 .. 1653  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
475 .. 1653  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
Cycle 3 GFP
2865 .. 3563  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2865 .. 3563  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
BleoR
3564 .. 3941  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
3564 .. 3941  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
AmpR
5074 .. 5934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5074 .. 5865  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5074 .. 5934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5866 .. 5934  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5074 .. 5934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4315 .. 4903  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4315 .. 4903  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
2159 .. 2462  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2159 .. 2462  =  304 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
1904 .. 2128  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1904 .. 2128  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
2463 .. 2666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2463 .. 2666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
3955 .. 4089  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3955 .. 4089  =  135 bp
SV40 polyadenylation signal
AmpR promoter
5935 .. 91  =  105 bp
AmpR promoter
5935 .. 91  =  105 bp
EM7 promoter
2784 .. 2831  =  48 bp
synthetic bacterial promoter
EM7 promoter
2784 .. 2831  =  48 bp
synthetic bacterial promoter
T7 promoter
1670 .. 1688  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1670 .. 1688  =  19 bp
promoter for bacteriophage T7 RNA polymerase
EF-1α intron A
706 .. 1644  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
706 .. 1644  =  939 bp
intron upstream of the start codon of human EF-1α
MCS
1715 .. 1886  =  172 bp
multiple cloning site
MCS
1715 .. 1886  =  172 bp
multiple cloning site
ORF:  1031 .. 1468  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  2102 .. 2356  =  255 bp
ORF:  84 amino acids  =  9.5 kDa
ORF:  5204 .. 5470  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1110 .. 1343  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  2850 .. 3941  =  1092 bp
ORF:  363 amino acids  =  40.5 kDa
ORF:  773 .. 1393  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  3464 .. 3847  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
ORF:  4064 .. 4345  =  282 bp
ORF:  93 amino acids  =  10.2 kDa
ORF:  2545 .. 2805  =  261 bp
ORF:  86 amino acids  =  9.3 kDa
ORF:  5074 .. 5934  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pTracer-EF V5-His A.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.